HPLC and mass spectrometry require increasingly complex sample preparation processes.
However, preparing samples manually wastes a lot of time and can compromise sample quality, directly affecting the final analysis results.
The Andrew system performs concentration normalization, serial dilution and other pipetting operations with a large variety of liquid properties. Andrew DOMINOs™ can be used with your HPLC vials without modification. You can also put Andrew in a chemical fume hood when you need to use volatile solvents.
Waters® and Andrew Alliance cooperate in the automation and validation of the GlycoWorks RapiFluor-MS Package®, demonstrating that – beyond significant operator time savings-the Andrew robot’s automation of the GlycoWorks with RFMS kit offers an easy, reliable and robust solution to release N-glycan analysis. N-Glycan analysis is routinely performed for biotherapeutic protein characterization. A recently introduced Waters® N-Glycan analysis kit using RapiFluor-MS (RFMS) labeling provides time savings over reductive amination labeling methods while also providing enhanced fluorescence (FLR) and mass spectrometry (MS) responses. The manual RFMS protocol includes two heating and cooling steps during protein denaturation and de-N-Glycosylation that have been achieved inside a DOMINO, and the solid phase extraction is also integrated in the process.